Steps To Follow.
1. Open the site to configure in IIS. Right click and select "Properties"
2. Click the HTTP Headers Tab, select “File Types” under the MIME Map section, and then click "New Type". Enter the following:
1. Associated Extension box: .flv
2. MIME Type box: flv-application/octet-stream
3. Click "OK" and close the IIS Properties box
4. You may need to restart IIS( start->run->iisreset).